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Dana-Farber Flow Cytometry - Hematologic Neoplasia and Jimmy Fund Cores

Dana-Farber Flow Cytometry
HEMATOLOGIC NEOPLASIA AND JIMMY FUND CORES

Resources


Analysis Software

FlowJo
FCSExpress
BD FACSDiva
OMIQ

Cytometry Groups

International Society for Advancement of Cytometry
New England Cytometry Users Group
​Purdue Flow Cytometry

Education

BD Flow Cytometry Training
Thermo Fisher Fluorescence Training
FlowJo University Tutorials
FCS Express Tutorials​​

Experiment Design

BD Spectrum Viewer
eBiosciences Spectra Viewer 
Biolegend Spectra Analyzer
Thermo Fisher SpectraViewer
Chromocyte

Instrument Parameters

M Aria III Laser Chart

M  BD Symphony S6 Laser Chart

JF Aria III Laser Chart

JF BD Symphony S6 Laser Chart
M BD LSRFortessa Laser Chart

​JF BD LSRFortessa Laser Chart

Protocols

Cell Surface Staining Protocol
Intracellular Staining Protocol
​Whole Blood Lysis Protocol
Ultracomp Bead Staining Protocol

Recommended Supplies

Ultracomp ​​Compensation Beads

Spherotech 8 Peak QC Beads

5 mL Snap Cap Tubes

​
5 mL 35um Filter Cap Tubes


15 mL Conical Tubes

​

1.5 mL LoBind Eppendorf Tubes​
96 Well Culture Plates


96 Well PCR Plates
384 Well Culture Plates


​384 Well PCR Plates

Vendors

Becton Dickinson

Cell Signaling Technologies
Invitrogen

Fluorofinder
​Sony 
Miltenyi
Biolegend

​User Guides

BD FACSAria II User Guide
BD LSRFortessa User Guide
BD HTS User Guide
​BD FACSDiva 8.0 Manual


©2017 Dana-Farber Flow Cytometry at Dana-Farber Cancer Institute