Cell Analysis

The Dana Farber Flow Core offers self run analysis on 7 cell analyzers, with a staff of expert cytometry technicians to assist with your experiments. Please follow the instructions below to optimize your analysis. See the Rates for Analysis, Analyzer Schedule, and Instruments pages for more information.

Analyzer Training
Analyzer Training is available for all new users, as well as experienced users who need retraining.
Please go to the New Users page for more information. 

Sample Preparation
Samples MUST BE FILTERED no more than 20 minutes before the start of your reservation. Please use 35 mm filter cap FACS tubes if available. 
Please prepare you sample at a concentration of 1 million/mL or less. Your sample can be prepared in any media or PBS. 
Cells can be analyzed using 5 mL FACS tubes, 96 well plates, or 384 well plates.

Analysis Assistance
The flow core staff are available for assistance with your experiments if you call the core 24 hours in advance it is greatly appreciated.
We ask your cooperation so that we will be able to assign a staff member to the assistance.

Lentivirus and Human Primary Cells
Analyzing Lentivirus and Human Primary Cells
  • ​ Cells must be analyzed in a 96 or 384 well plate on the HTS plate reader.
  • Researcher is responsible for running an extended cleaning procedure and disinfecting the analyzer area.
  • The researcher is responsible for removal and disposal of gloves, and all wipes used to disinfect the analyzer and affected areas.

Read the full Lentivirus Analysis protocol here. 
Read the full Human Primary Cell Analysis protocol here.