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Dana-Farber Flow Cytometry - Hematologic Neoplasia and Jimmy Fund Cores

Dana-Farber Flow Cytometry
HEMATOLOGIC NEOPLASIA AND JIMMY FUND CORES

Hematologic Neoplasia Core Instruments



Cell Sorters

Location: M307

Cell Sorting Requirements
The Hematologic Neoplasia Flow Cytometry Facility operates  a Symphony S6 and  BD FACSAria II high speed cell sorters housed in biosafety cabinets, capable of sorting up to 4 populations simultaneously, ​as well as into single cell plates. 
Picture
M Symphony S6
element_settings.Image_30621876.default

M Aria III SORP


Violet
Blue
Yellow Green
​Red
   ​405 nm
​   488 nm
​   561 nm
​   641 nm
Violet
Blue
Yellow Green
Red
405 nm
​488 nm
​561 nm
641 nm
        4 Laser System
 16 Parameter
   M Symphony S6 Laser Chart
 6 Way Sorting
                                 4 Laser System
17 Parameters
M Aria III Laser Chart
4 Way Sorting

Acquisition

​​5 mL FACS Tubes or 15 mL Conical Tubes  

Collection

1.5 mL Eppendorf Tubes, 5 mL FACS Tubes, 15 mL Conical Tubes 
​           6, 24, 48, 96, or 384 Well Plates
                      

Cell Analyzers
Location: M583, and M584

Analysis Requirements
The Hematologic Neoplasia Core maintains and trains users on 4 BD flow cytometers for self run use.
For training, please refer to the New Users page.
Picture

BD LSR Fortessa


Instruments

M Fortessa 1 - M584                            
M Fortessa 2 - M584                            
M Fortessa 3 - M583             
M Fortessa X-20 - M583           


Specifications

4 Laser System
Violet
Blue
Yellow Green
​
Red
405 nm
​488 nm
​561 nm
​641 nm
​          17 Parameters
   M Fortessa Laser Chart

Acquisition

5 mL FACS Tubes                     
96 or 384 Well Plates                   


©2017 Dana-Farber Flow Cytometry at Dana-Farber Cancer Institute