Cell Sorting-
Please use the Scheduling Smartsheet Request Form to schedule a sorting reservation
The Dana Farber Flow Core offers cell sorting performed by experienced cytometry technicians. Please follow the instructions below to optimize your sorts. See the Rates for Sorting, Sorter Schedule, and Instruments pages for more information.
Reference the "Before You Sort" PDF for all things sorting at DFCI Flow Core
Sample Preparation |
Samples MUST BE FILTERED no more than 30 minutes before the start of your reservation. Please use 35 mm filter cap FACS tubes if available.
Please prepare your cells at the appropriate concentration:
Cells can be prepared in any media or PBS. Please bring collection tubes with media or buffer in the bottom. Controls are required for the technicians to properly set up your sort. Please bring a negative/unstained control, and single color controls for each fluorochrome in your experiment. |
Nozzles |
The nozzle generates droplets containing cells that the instrument then sorts. We offer two different nozzle sizes:
70 µm Nozzle
100 µm Nozzle
Please select the nozzle you are using on the iLabs reservation sheet so we can set up before you arrive if time allows. |
Sort Collection |
Sorted cells can be collected into:
Please make sure to have media in your collection tubes, and choose collection tubes based on the number of cells you expect to get back per sample and the nozzle you are using: 70 µm Nozzle
100 µm Nozzle
Note: The number of cells that are collected into the tube are usually 60 - 80% of the number that the instrument reports as sorted. Please take this into consideration when planning your experiments. |
Lentivirus and Human Primary Cells |
Lentivirus and Human Primary Cells
Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells, so they are one of the most efficient methods of a gene delivery vector. HIV, SIV, and FIV are all examples of lentiviruses. Sorting Lentivirus and Human Primary Cells Requirements:
Read the full Human Primary Sorting protocol here. |
Sorter Training |
Sorter training for the Sony MA900 is available for all users. Please contact the core for more information.
Aria Sorter training is currently limited to researchers that require after hours access for mouse or patient samples. Please contact the core for more information about being trained on our sorters. |