Jimmy Fund Core Instruments

Cell Sorters

Location: M307
The DFCI Jimmy Fund Flow Cytometry Core operates 1 BD FACSAria II high speed cell sorter housed in biosafety cabinet, capable of sorting up to 4 populations simultaneously, as well as into single cell plates. We also house 2 Sony MA900s and a Sony SH800 cell sorter. 
JF Symphony S6 UV

      JF Aria III
JF  SONY MA900
5 Laser System
4 Laser System
Ultra Violet
Violet
Blue
Yellow Green
​Red
355 nm
​405 nm
​488 nm
​561 nm
​641 nm
Violet
Blue
Yellow Green
​Red
405 nm
​488 nm
​561 nm
​641 nm
 23 Parameters
JF Symphony  S6 UV
Laser Chart
​
6-Way Sorting
17 Parameters 
JF Aria III Laser Chart


​4-Way Sorting
4 Laser Colinear System​
Violet
Blue
Yellow Green
Red
​
405 nm
​488 nm
​561 nm
​641 nm
12 Parameters 
​MA900 Laser Chart

 
​4-Way Sorting

Acquisition
​​5 mL FACS Tubes or 15 mL Conical Tubes      ​                    

Collection
1.5 mL Eppendorf Tubes, 5 mL FACS Tubes, 15 mL Conical Tubes,                     
​6, 24, 48, 96, or 384 Well Plates         ​

Cell Analyzers
Location: M307
The Jimmy Fund core maintains and trains users on 3 BD LSRFortessa analyzers, for self run use. For training, please refer to the New Users page. 

BD LSR Fortessa 
SONY ID7000 Spectral Analyzer

Instruments
JF Fortessa B
JF Fortessa C
Mayer 309 ID7000-Alpha
Mayer 307 ID7000-Bravo

Specifications
4 Laser System
Violet
Blue
Yellow Green
Red
405 nm
​488 nm
​561 nm
​641 nm
JF Fortessa B/C - 17 Parameters
JF Fortessa Laser Chart
                5 Laser System
     Ultra Violet
​     Violet
     Blue
     Yellow Green
     Red
355 nm
​405 nm
​488 nm
​561 nm
​641 nm
  40 Parameter Spectral Analyzer

Acquisition
​​5 mL FACS Tubes
96 or 384 Well Plates (Fortessa B/C Only)