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Hematologic Neoplasia Core Instruments

 

Cell Sorters

Location: M307
Cell Sorting Requirements
 

The Hematologic Neoplasia Flow Cytometry Facility operates a Symphony S6 (6-way sorting) and  BD FACSAria III (4-way) High Speed Cell Sorters housed in biosafety cabinets, capable of sorting bulk ​as well as into single cell plates. 

BD Symphony S6 & BD Symphony S6 UV

M Symphony S6

Violet 405 nm
Blue 488 nm
Yellow Green 561 nm
Red 641 nm

4 Laser System

16 Parameter

Instrument Laser Configuration

6 Way Sorting

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M Aria III SORP

Violet 405 nm
Blue 488 nm
Yellow Green 561 nm
Red 641 nm

4 Laser System

17 Parameter

Instrument Laser Configuration

4 Way Sorting


Acquisition

5 mL FACS Tubes or 15 mL Conical Tubes


Collection

1.5 mL Eppendorf Tubes, 5 mL FACS Tubes, 15 mL Conical Tubes 6, 24, 48, 96, or 384 Well Plates


 

Cell Analyzers

Location: M307
Analysis Requirements

The Hematologic Neoplasia Core maintains and trains users on 4 BD flow cytometers for self run use.
For training, please refer to the New Users page.
 

BD LSR Fortessa (6)

BD LSR Fortessa


Instruments

M Fortessa 1 - M307
M Fortessa 2 - M307
M Fortessa 3 - M307
M Fortessa X-20 - M307


Specifications

4 Laser System

Violet                 405 nm
Blue                   ​488 nm
Yellow Green     ​561 nm
​Red                   ​ 641 nm

17 Parameters
 Instrument Laser Configuration 


Acquisition

5 mL FACS Tubes
96 or 384 Well Plates